Guide for Purchasing Sequencing: 7th Revised Version
The Sequencing Buyer's Guide, now in its latest edition, provides a valuable resource for researchers, clinicians, and industry professionals seeking to navigate the ever-evolving world of sequencing technology. The guide explores the current trends and technologies in sequencing, including Next-Generation Sequencing (NGS), emerging methods like Roche's Sequencing by Expansion (SBX), and market growth forecasts focusing on applications like oncology and rare disease diagnostics.
The guide is organized around sequencing technologies, library preparation protocols, market segments, applications, vendor platforms, consumables, and performance metrics. Notable contributors include Pankaj Agrawal, Chief of Neonatology at Holtz Children's Hospital and Jackson Health System, and Rosie Bamford, a Postdoctoral Research Fellow in the Complex Disease Epigenomics Group at the University of Exeter.
The first chapter focuses on the first steps in sample preparation, specifically nucleic acid extraction. The second chapter highlights both DNA and RNA library preparation kits for short-read and long-read systems in the final step of sample preparation, library preparation. The third chapter overviews the current commercial selection of short-read sequencing platforms, with information on Roche's new sequencing by expansion technology provided at the end of this chapter.
The guide also delves into the development of long-read sequencing technologies, allowing for exploration of genomes at unprecedented resolutions. It discusses the challenges and ethical hurdles of using clinical data in genomic medicine, with newborn screening as an example. The guide further explores the option of outsourcing sequencing experiments to external groups, departments, or companies.
To offer a more comprehensive understanding, the guide includes interviews and panel discussions with experts across various sectors, including academia, pharmaceuticals, and clinics. It provides up-to-date specifications of sequencing instrumentation and the reagents used to prepare samples for NGS, displayed side by side for comparison. The guide also highlights key single-cell, spatial, and multi-omics sequencing technologies.
The guide is now available for download, offering a valuable resource for anyone interested in staying informed about the latest developments in the field of sequencing technology. For detailed information about the "Sequencing Buyer’s Guide 2025," including its contributors and chapter list, it is recommended to check directly with major sequencing technology providers or industry publishers who produce such buyer's guides.
- The guide delves into the development of long-read sequencing technologies, allowing for exploration of genomes at unprecedented resolutions.
- The first chapter focuses on the first steps in sample preparation, specifically nucleic acid extraction.
- The guide provides up-to-date specifications of sequencing instrumentation and the reagents used to prepare samples for NGS, displayed side by side for comparison.
- The sequencing technologies, library preparation protocols, market segments, applications, vendor platforms, consumables, and performance metrics are organized around in the guide.
- Notable contributors include Pankaj Agrawal, a clinician, and Rosie Bamford, a researcher in the field of genomic medicine.
- The guide offers a valuable resource for researchers, clinicians, and industry professionals seeking to navigate the ever-evolving world of sequencing technology, with an emphasis on technologies like Next-Generation Sequencing (NGS), and applications such as oncology and rare disease diagnostics.
